Thisrabbitpolyclonalantibodywasraisedagainstfusionproteinandrecognizeshuman,mouseandratATPBindingCassetteSubfamilyDMember2(ABCD2).
Highlights:
- Reactswithhuman,mouseandratABCD2
- SuitableforWesternBlotandImmunofluorescenceandImmuneElectronMicroscopyapplications
ABCD2isamemberofthesuperfamilyofATP-bindingcassette(ABC)transporters.ABCproteinstransportvariousmoleculesacrossextra-andintra-cellularmembranes.ABCgenesaredividedintosevendistinctsubfamiliesandthisproteinisamemberoftheALDsubfamily,whichisinvolvedinperoxisomalimportoffattyacidsand/orfattyacyl-CoAsintheorganelle.DiseasesassociatedwithABCD2includeAdrenoleukodystrophyandDemyelinatingDisease.
FromthelaboratoryofGregA.Graf,PhD,UniversityofKentucky.
| ProductType: | Antibody |
| Antigen: | ABCD2peptide |
| AccessionID: | GeneID:26874 |
| MolecularWeight: | 78kDa |
| Clonality: | Polyclonal |
| Reactivity: | Mouse,RatandHuman |
| Immunogen: | FusionPeptide |
| SpeciesImmunized: | Rabbit |
| Epitope: | Aa377-711 |
| Buffer: | 50%glycerol,50mMtris(pH7.4)and0.01%azide |
| TestedApplications: | WB(1:2000),IF(1:200),IEM(1:50) |
| Storage: | -80C |
| Shipped: | DryIce |
ImmunofluorescenceMicroscopyandImmunoblotAnalysisofD2ProteininCulturedCells.
(A)CellsweretransfectedwithaplasmidcontainingaCDNAencodingmouseD2.CellswereprocessedforindirectimmunofluorescencemicroscopyusingthepolyclonalD2antibody.Inset:lysatesfromcellstransfectedwithcontrolplasmid(V)andD2wereanalyzedbySDS-PAGEandimmunoblotting.(B)TotalmembraneswerepreparedfromADIposecollectedfromwild-typeandD2-deficient(D2-/-)miceandanalyzedbyimmunoblottingwiththeD2antibody.
- LiuJ,SabevaNS,BhatnagarS,LiXA,PujolA,GrafGA.ABCD2isabundantinadiposetissueandopposestheaccumulationofdietaryerucicacid(C22:1)infat.JLipidRes.2010Jan;51(1):162-8.
- LiuJ,LiangS,LiuX,BrownJA,NewmanKE,SunkaraM,MorrisAJ,BhatnagarS,LiX,PujolA,GrafGA.TheabsenceofABCD2sensitizesmicetodisruptionsinlipidmetabolismbydietaryerucicacid.JLipidRes.2012Jun;53(6):1071-9.
- LiuX,LiuJ,LesterJD,PijutSS,GrafGA.ABCD2identifiesasubclassofperoxisomesinmouseadiposetissue.BiochemBiophysResCommun.2015Jan2;456(1):129-34.
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