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Kerafast/Anti-ADAM17 (mouse), Cytoplasmic Domain Antibody/EHS008/100uL
ThisrabbitIgGpolyclonalantibodywasgeneratedagainsttheGST-ADAM17-cytoplasmictailandisspecificforthecytoplasmicdomainofmouseADAM17.
TheADAM(ADisintegrinAndMetalloproteaseDomain)familyisauniquegroupofproteinsthathastheABIlitytocleaveorshedextracellularportionsoftransmembraneproteins.Inaddition,ADAMsareinvolvedincell-cellandcell-matrixinteractions(e.g.,fertilization,muscledevelopmentandneurogenesis).
ADAM17(alsoreferredtoasTumorNecrosisFactorConvertase,TACE)isinvolvedintheprocessingoftumornecrosisfactoralpha(TNF-a)atthesurfaceofthecell,andisalsorequiredforsheddingseveralligandsoftheepidermalgrowthfactorreceptorandmanyothermembraneproteins.
FromthelaboratoryofCarlP.Blobel,MD,PhD,HospitalforSpecialSurgery.
PartofTheInvestigator"sAnnexeprogram.
| ProductType: | Antibody |
| Antigen: | GST-ADAM17CytoplasmcDomain |
| MolecularWeight: | ~100kDa(matureform),~120kDa(pro-form) |
| Isotype: | IgG |
| Clonality: | Polyclonal |
| Reactivity: | Mouse |
| Immunogen: | GST-cytocorrespondingtothecytoplasmicdomainofmouseADAM17 |
| SpeciesImmunized: | Rabbit |
| Epitope: | Cytoplasmicdomain |
| PurificationMethod: | Serum,notpurified |
| TestedApplications: | WB(1:1000) |
| Storage: | -80C |
| Shipped: | Dryice |
| Antibody | SpeciesReactivity | Specificity |
| ADAM9 | Human | CytoplasmicDomain |
| ADAM9 | Mouse | CytoplasmicDomain |
| ADAM12 | Mouse | CytoplasmicDomain |
| ADAM15 | Human | CytoplasmicDomain |
| ADAM15 | Mouse | CytoplasmicDomain |
| ADAM15 | Human | ExtracellularDomain |
| ADAM15 | Mouse | DisintegrinDomain |
| ADAM17 | Mouse | CytoplasmicDomain |
| ADAM17 | Mouse | Prodomain |
| ADAM19 | Mouse | CytoplasmicDomain |
| ADAM28 | Mouse | CytoplasmicDomain |
| ADAM28 | Mouse | ExtracellularDomain |
| SNX9 | Mouse | CytoplasmicDomain |
WesternBlotAnalysis
Antibodiesagainst:pro-domainfusedtoGST(α-PRO),cytoplasmicdomainfusedtoGST(α-CYTO).
Adaptedfrom:SchlöndorffJ,etal.JBiochemJ.2000,347,131-138.
Celllysisbuffermustcontain5mM1,10PhenanthrolinetopreventautodegradationofthematureformofADAM17,whichisnotpreventedbyEDTAortypicalproteaseinhibitorcocktails(Schlöndorffetal.,BiochemJ.2000,347,131-138).
- Schlöndorff,J.;Becherer,J.D.;Blobel,C.P.Intracellularmaturationandlocalizationofthetumournecrosisfactoralphaconvertase(TACE).BiochemJ.2000,347,131-138.
- Zheng,Y.;Schlondorff,J.;Blobel,C.P.Evidenceforregulationofthetumornecrosisfactoralpha-convertase(TACE)byprotein-tyrosinephosphatasePTPH1.J.Biol.Chem.2002,277,42463-42470.
- Horiuchi,K.;KimuraT.;MiyamotoT.;TakaishiH.;OkadaY.;Toyama,Y.,Blobel,C.P.Cuttingedge:TNF-alpha-convertingenzyme(TACE/ADAM17)inactivationinmousemyeloidcellspreventslethalityfromendotoxinshock.J.Immunol.2007,179,2686-2689.
- LeGall,S.M.,Maretzky,T.,Issuree,P.D.,Niu,X.D.;Reiss,K.,Saftig,P.,Khokha,R.,Lundell,D.,Blobel,C.P.ADAM17isregulatedbyarapidandreversiblemechanismthatcontrolsaccesstoitscatalyticsite.J.Cell.Sci.2010,123,3913-3922.
- Maretzky,T.,Evers,A.,Zhou,W.,Swendeman,S.L.,Wong,P.M.,Rafii,S.,Reiss,K.,BlobelC.P.Migrationofgrowthfactor-stimulatedepithelialandendothelialcellsdependsonEGFRtransactivationbyADAM17.Nat.Commun.2011,2,1-25.
- McIlwainDR,LangPA,MaretzkyT,HamadaK,OhishiK,ManeySK,BergerT,MurthyA,DuncanG,XuHC,LangKS,HäussingerD,WakehamA,Itie-YoutenA,KhokhaR,OhashiPS,BlobelCP,MakTW.iRhom2regulationofTACEcontrolsTNF-mediatedprotectionagainstListeriaandresponsestoLPS.Science.2012Jan13;335(6065):229-32.
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