ThesemousemonoclonalantibodiesweregeneratedagainstArABIdopsisthalianaActin-7andrecognizeA.thalianaActin-7.
Highlights:
- ReactwithA.thalianaActin-7(Maydisplayreactivitytowardmultipleotherespecies)
- SuitableforWesternBlot,ELISAandImmunofluorescenceapplications
Actin-7hasbeenidentifiedinanimals,plantsandprotists.Duringplantgrowthanddevelopment,thephytohormoneauxininducesawidearrayofchangesthatincludecelldivision,cellexpansion,celldifferentiation,andorganinitiation.IthasbeensuggestedthattheactinCytoskeletonplaysanactiveroleintheelaborationoftheseresponsesbydirectingspecificchangesincellmorphologyandcytoarchitecture.ThepromoterandproteinproductofACT7,anAraBDopsisvegetativeactingene,arerapidlyandstronglyinducedinresponsetoexogenousauxin.ACT7isrequiredfortheformationofcallustissue,andisalsoessentialforgerminationandrootgrowthACT7actingeneisalsoexpressedinrapidlydevelopingtissuesandrespondstoexternalstimuli,suchasexposuretohormones.
FromthelaboratoryofRichardB.Meagher,PhD,UniversityofGeorgia.
| ProductType: | Antibody |
| Name: | Actin-7Antibodies |
| AccessionID: | P53492 |
| Antigen: | Actin-7 |
| Isotype: | IgG |
| Clonality: | Monoclonal |
| CloneName: | 29G12.G5.G6,33E8.C11.F5.D1,36H8.C12.H10.B6 |
| Reactivity: | TestedinA.thaliana.Maydisplayreactivitytowardmultipleotherespecies(ND) |
| Immunogen: | A.thalianaActin-7 |
| SpeciesImmunized: | BALB/cmouse |
| PurificationMethod: | ProteinG |
| Buffer: | 0.1MSodiumPhosphate,pH7.4,0.15MNaCl,0.05%(w/v)SodiumAzide |
| TestedApplications: | WB,ELISA,IF(ItisadvisabletouseallthreeMAbsinthefirst-time,qualitativeexperimentalsetuptodeterminewhichisthemostsuitableforaspecificexperiment.ThesemaybeusedyocomplementexcperimentscarriedoutinvolvingmAbGEauniversalanti-actinsantibody.) |
| Storage: | -20C |
| Shipped: | Coldpacks |
- Kandasamy,M.K.,Gilliland,L.U.,McKinney,E.C.,andMeagher,R.B.(2001).Oneplantactinisovariant,ACT7,isinducedbyauxinandrequiredfornormalcallusformation.PlantCell13,1541-54.
- Kandasamy,M.K.,McKinney,E.C.,andMeagher,R.B.(1999).Thelatepollen-specificactinsinangiosperms.PlantJ18,681-91.
- Kandasamy,M.K.,McKinney,E.C.,Roy,E.,andMeagher,R.B.(2012).Plantvegetativeandanimalcytoplasmicactinssharefunctionalcompetenceforspatialdevelopmentwithprotists.PlantCell24,2041-57.
- L.U.Gilliland,L.C.Pawloski,M.K.Kandasamy,andR.B.Meagher,ArabidopsisactingeneACT7playsanessentialroleingerminationandrootgrowth,PlantJournal,vol.33,no.2,pp.319328,2003.
- McDowell,JM,An,Y-q,Huang,S,McKinney,EC,MeagherRB:TheArabidopsisACT7ActinGeneIsExpressedinRapidlyDevelopingTissuesandRespondstoSeveralExternalStimuli.PlantPhysiol.(1996)111:699-711.
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