ThisrabbitIgGpolyclonalantibodywasgeneratedagainsttheGST-ADAM15-cytoplasmictailandisspecificforthecytoplasmicdomainofmouseADAM15.
TheADAM(ADisintegrinAndMetalloproteaseDomain)familyisauniquegroupofproteinsthathastheABIlitytocleaveorshedextracellularportionsoftransmembraneproteins.Inaddition,ADAMsareinvolvedincell-cellandcell-matrixinteractions(e.g.,fertilization,muscledevelopmentandneurogenesis).
ADAM15(Metargidin)isamembrane-anchoredmetalloproteinasethathasbeenimplicatedinpathologicalneovascularizationandprostatecancermetastasis.
FromthelaboratoryofCarlP.Blobel,MD,PhD,HospitalforSpecialSurgery.
PartofTheInvestigator"sAnnexeprogram.
| ProductType: | Antibody |
| AccessionID: | AF006196 |
| Antigen: | GST-ADAM15cytoplasmicdomain |
| MolecularWeight: | ~100kDa |
| Isotype: | IgG |
| Clonality: | Polyclonal |
| Reactivity: | Mouse |
| Immunogen: | GST-cytocorrespondingtothecytoplasmicdomainofmouseADAM15 |
| SpeciesImmunized: | Rabbit |
| Epitope: | Cytoplasmicdomain |
| PurificationMethod: | Serum,notpurified |
| TestedApplications: | WB(1:500) |
| Storage: | -80C |
| Shipped: | Dryice |
| Antibody | SpeciesReactivity | Specificity |
| ADAM9 | Human | CytoplasmicDomain |
| ADAM9 | Mouse | CytoplasmicDomain |
| ADAM12 | Mouse | CytoplasmicDomain |
| ADAM15 | Human | CytoplasmicDomain |
| ADAM15 | Mouse | CytoplasmicDomain |
| ADAM15 | Human | ExtracellularDomain |
| ADAM15 | Mouse | DisintegrinDomain |
| ADAM17 | Mouse | CytoplasmicDomain |
| ADAM17 | Mouse | Prodomain |
| ADAM19 | Mouse | CytoplasmicDomain |
| ADAM28 | Mouse | CytoplasmicDomain |
| ADAM28 | Mouse | ExtracellularDomain |
| SNX9 | Mouse | CytoplasmicDomain |
WesternBlotAnalysis
WesternblotofreducedglycoproteinsfromtheindicatedtissueswereprobedwithADAM15cytoplasmicdomainantibody.
Adaptedfrom:LumL,etal.J.BiolChem.1998,273,26236-26247.
- LumL.;Reid,M.S.;Blobel,C.P.IntracellularmaturationofthemousemetalloproteasedisintegrinMDC15.J.BiolChem.1998,273,26236-26247.
- HoriuchiK,WeskampG,LumL,HammesHP,CaiH,BrodieTA,LudwigT,ChiusaroliR,BaronR,PreissnerKT,ManovaK,BlobelCP.PotentialroleforADAM15inpathologicalneovascularizationinmice.MolCellBiol.2003Aug;23(16):5614-24.
- MaretzkyT,YangG,OuerfelliO,OverallCM,WorpenbergS,HassiepenU,EderJ,BlobelCP.Characterizationofthecatalyticactivityofthemembrane-anchoredmetalloproteinaseADAM15incell-basedassays.BiochemJ.2009Apr28;420(1):105-13.
- MaretzkyT,LeGallSM,Worpenberg-PietrukS,EderJ,OverallCM,HuangXY,PoghosyanZ,EdwardsDR,BlobelCP.Srcstimulatesfibroblastgrowthfactorreceptor-2sheddingbyanADAM15splicevariantlinkedtobreastcancer.CancerRes.2009Jun1;69(11):4573-6.
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